𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Retinoic acid induced growth arrest of human breast carcinoma cells requires protein kinase Cα expression and activity

✍ Scribed by Yunhi Cho; Ann P. Tighe; David A. Talmage

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
227 KB
Volume
172
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Retinoic acid inhibits proliferation of hormone-dependent, but not hormone-independent breast cancer cells. Retinoic acid-induced changes in cellular proliferation and differentiation are associated with disturbances in growth factor signaling and frequently with changes in protein kinase C expression. PKC delta, epsilon, and zeta are expressed in both hormone-dependent (T-47D) and hormone-independent (MDA-MB-231) cell lines. Retinoic acid arrested T-47D proliferation, induced PKC alpha expression and concomitantly repressed PKC zeta expression. The changes in PKC alpha and PKC zeta reflect retinoic acid-induced changes in mRNA. In contrast, retinoic acid had no effect on growth, or PKC expression in MDA-MB-231 cells. Growth arrest and the induction of PKC alpha, but not the reduction in PKC zeta, resulted from selective activation of RAR alpha. In total, these results support an important role for PKC alpha in mediating the anti-proliferative action of retinoids on human breast carcinoma cells.

📜 SIMILAR VOLUMES

Ornithine decarboxylase over-expression
Ornithine decarboxylase over-expression stimulates mitogen-activated protein kinase and anchorage-independent growth of human breast epithelial cells
✍ Andrea Manni; Rita Wechter; Susan Gilmour; Michael F. Verderame; David Mauger; L 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 140 KB

In these experiments we tested the hypothesis that constitutive activation of polyamine(PA) biosynthesis may contribute to mammary carcinogenesis. Spontaneously immortalized normal human MCF-10A breast epithelial cells were infected with the retroviral vector pLOSN containing a cDNA which codes for

Participation of type II protein kinase
Participation of type II protein kinase A in the retinoic acid-induced growth inhibition of SH-SY5Y human neuroblastoma cells
✍ Se Nyun Kim; Sang Gyun Kim; Sang Dai Park; Yoon S. Cho-Chung; Seung Hwan Hong 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 228 KB

To examine the role of protein kinase A (EC 2.7.1.37) isozymes in the retinoic acid-induced growth inhibition and neuronal differentiation, we investigated the changes of protein kinase A isozyme patterns in retinoic acid-treated SH-SY5Y human neuroblastoma cells. Retinoic acid induced growth inhibi

Inhibition of cyclin-dependent kinases 2
Inhibition of cyclin-dependent kinases 2 and 4 activities as well as induction of cdk inhibitors p21 and p27 during growth arrest of human breast carcinoma cells by (−)-epigallocatechin-3-gallate
✍ Yu-Chih Liang; Shoei-Yn Lin-Shiau; Chieh-Fu Chen; Jen-Kun Lin 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 160 KB 👁 2 views

Ϫ)-Epigallocatechin-3-gallate (EGCG) potently inhibits cell proliferation and suppresses tumor growth both in vitro and vivo, but little is known regarding the cell cycle regulatory proteins mediating these effects. This study investigated the effects of EGCG and other catechins on the cell cycle pr