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Uncovering of intracellular water in cultured cells

✍ Scribed by Jean-Philippe Galons; Silvia Lope-Piedrafita; Joseph L. Divijak; Curt Corum; Robert J. Gillies; Theodore P. Trouard

Book ID
102533654
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
498 KB
Volume
54
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

The complexity of biologic tissues, with multiple compartments each with its own diffusion and relaxation properties, requires complex formalisms to model water signal in most magnetic resonance imaging or magnetic resonance spectroscopy experiments. In this article, we describe a magnetic susceptibility‐induced shift in the resonance frequency of extracellular water by the introduction of a gadolinium contrast agent to medium perfusing a hollow fiber bioreactor. The frequency shift of the extracellular water (+185 Hz at 9.4 T) uncovers the intracellular water and allows direct measurement of motional and relaxation properties of the intracellular space. The proposed method provides a unique tool for understanding the mechanisms underlining diffusion and relaxation in the intracellular space. Magn Reson Med 54:79–86, 2005. © 2005 Wiley‐Liss, Inc.

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