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Ischemia-induced changes of intracellular water diffusion in rat glioma cell cultures

✍ Scribed by Theodore P. Trouard; Kevin D. Harkins; Joseph L. Divijak; Robert J. Gillies; Jean-Philippe Galons

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
254 KB
Volume
60
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

Diffusion‐weighted MRI is commonly used in the diagnosis and evaluation of ischemic stroke because of the rapid decrease observed in the apparent diffusion coefficient (ADC) of tissue water following ischemia. Although this observation has been clinically useful for many years, the biophysical mechanisms underlying the reduction of tissue ADC are still unknown. To help elucidate these mechanisms, we have employed a novel three‐dimensional (3D) hollow‐fiber bioreactor (HFBR) perfused cell culture system that enables cells to be grown to high density and studied via MRI and MRS. By infusing contrast media into the HFBR, signals from intracellular water and extracellular water are spectroscopically resolved and can be investigated individually. Diffusion measurements carried out on C6 glioma HFBR cell cultures indicate that ischemia‐induced cellular swelling results in an increase in the ADC of intracellular water from 0.35 μm^2^/ms to approximately 0.5 μm^2^/ms (diffusion time = 25 ms). Magn Reson Med 60:258–264, 2008. © 2008 Wiley‐Liss, Inc.

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