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Ultraviolet light-induced inhibition of small nuclear RNA synthesis

✍ Scribed by Brian P. Eliceiri; Kanakendu Choudhury; Quincy O. Scott; George L. Eliceiri

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 656 KB
Volume: 138
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041380320

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✦ Synopsis

Lorrrs Mizzouri 63 104

Two apparently distinct types of inhibition of the synthesis of U1, U2, U3, U4, and US small nuclear KNA, induced by ultraviolet (UV) radiation, have been described before: immediate and delaycd. O u r present observations can be summarized as follows: a) neither the immediate nor the delayed inhibition appear to be mediated by the formation of cyclobutane pyrimidine dimers, since they were not prevented by photoreactivating light, in ICR 2A frog cells; b) the inhibition of U1 RNA synthesis, monitored in HeLa cell5 within the first few minutes after irradiation, extrapolated to a substantial suppression at time zero of postirradiation cell incubation, providing further support for the proposal that the immediate inhibition is a reaction separate from the delayed UV light-induced inhibition of U1 RNA synthesis; c) the transition from the pattern of the immediate inhibition to that of the delayed inhibition (disappearanw of the LJV-resistant fraction of U1 RNA synthesis and increased rate of inhibition) occurred gradually, without an apparent threshold, within the first 2 hr of incubation after irradiation; and d) the incident UV dose that resulted in a 17% level of residual U1 RNA synthesis (D.17) during the delayed inhibition was about 7 J/m', with an apparent UV dose threshold, and was about 60 Jim2 for tht. immediate inhibition.

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