Ultraviolet detection of peptides by reversed-phase liquid chromatography using an in-line reactor containing copper metal

✍ Scribed by Hiroaki Kubo; Akiko Tsujimura; Akira Toriba; Toshio Kinoshita

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 307 KB
• Volume: 309
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(95)00088-h

✦ Synopsis

A general method has been developed for the selective ultraviolet detection of peptides using reversed-phase liquid chromatography.

The method is based on the formation of copper(peptide complexes by the reaction of peptides with copper(H) ions in an alkaline mobile phase through an in-line reactor containing copper metal. Amino acid, di-, tri-and tetra peptides used as model compounds (leucine, glycylleucine, leucylglycylglycine and alanylalanylalanylalanine) are separated on a column containing (&-bonded vinyl alcohol copolymer gel (Asahipak ODP-50, 5 pm, 150 mm X 6 mm id.) and an alkaline mobile phase (pH 11.0, 25 mM phosphate buffer). A detection limit of 200 pg (S/N = 3) was obtained for alanylalanylalanylalanine.