Coulometric detection of peptides by reversed-phase liquid chromatography with a solid-phase reactor containing copper metal

✍ Scribed by Hiroaki Kubo

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 295 KB
• Volume: 287
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)e0595-x

✦ Synopsis

Ah&act A general reversed-phase liquid chromatographic method with coulometric detection for the determination of peptides has been established using a solid-phase reactor containing copper metal. The method is based on the formation of electrochemically oxidizable copper(B)-peptide complexes by the reaction of peptides with copper(B) ions in an alkaline mobile phase through the solid-phase reactor. Di-and tripeptides used as model compounds (6 species) are separated on a column contained C,,-bonded vinyl alcohol copolymer gel (Asahipak ODP-50, $m, 250 mm x 6.0 mm i.d.) and an alkaline mobile phase (pH 11.0, 40 mM borate buffer). A detection limit of 5 ng was obtained for alanylalanylalanine.