Ultratrace analysis for trans,trans-muconic acid by electrophoric derivatization and capillary gas chromatography

✍ Scribed by Feng-Min Lin; Hsin-Lung Wu; Hwang-Shang Kou; Shun-Jin Lin

• Book ID: 104103028
• Publisher: Elsevier Science
• Year: 2002
• Tongue: English
• Weight: 98 KB
• Volume: 455
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(01)01573-2

✦ Synopsis

A highly sensitive method is described for the determination of trans,trans-muconic acid (MA), a biomarker of benzene exposure. The method is based on the derivatization of MA with an electrophoric reagent, 2-(pentafluorophenoxy)ethyl-2-(piperidino)ethanesulfonate, using potassium carbonate and 18-crown-6 ether as reaction activators. The resulting pentafluorophenoxy derivative of MA was analyzed by capillary GC with an electron-capture detector (ECD). The lower quantitation limit of the method is attainable at 0.3 M of MA with a detection limit of about 60 nM (S/N = 3) (60 fmol per 1.0 l injection). Application of the method to the analysis of MA in urine proved feasible.