Electrophore-labeling and alkylation of standards of nucleic acid pyrimidine bases for analysis by gas chromatography with electron-capture detection
✍ Scribed by Albert Nazareth; Markus Joppich; Samy Abdel-Baky; Kathleen O'Connell; Abdellah Sentissi; Roger W. Giese
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 704 KB
- Volume
- 314
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
✦ Synopsis
The pyrimidine bases cytosine, uracil and thymine, along with some analogues, are electrophore-labeled either with pentafluorobenzoyl chloride (PFBC), pentafluorophenylsulfonyl chloride (PPSC), or heptafluorobutyric anhydride. Subsequent alkylation is most successful for PFB-cytosine, PPS-uracil, and PPS-thymine. These same alkylated compounds also have the highest aqueous stability and respond most strongly by gas chromatography-electron-capture detection. One of these derivatives, determined to be N4-PFB-1,3-dimethylcytosine by authentic synthesis, and its 5methyl analogue, can be detected with good precision down to the lOO-fg level. Poor reproducibility is encountered at the IO-fg level.