Ultra-performance LC MS/MS method for quantification of clopidogrel active metabolite

✍ Scribed by Xavier Delavenne; Thierry Basset; Paul Zufferey; Nora Malouk; Silvy Laporte; Patrick Mismetti

• Book ID: 102446078
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 155 KB
• Volume: 33
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201000115

✦ Synopsis

Abstract

A sensible ultra‐performance LC–MS method was developed and validated for the quantification of clopidogrel active metabolite in human plasma, with clopidogrel D4 as internal standard. Plasma pretreatment involved a one‐step protein precipitation with acetonitrile. The separation was performed by reverse‐phase chromatography on a C8 column. The method was linear over the concentration range of 1–150 ng/mL. The intra‐ and inter‐day precision values were below 17% and accuracy was from 1.7 to 7.5% at all quality control levels. The lower LOQ was 0.8 ng/mL. Sample analysis time was reduced to 5 min including sample preparation (limited to protein precipitation). The present method was successfully applied to a clopidogrel active metabolite pharmacokinetic study following oral administration to healthy volunteers.