Type I collagen synthesis and degradation in peritendinous tissue after exercise determined by microdialysis in humans
✍ Scribed by Henning Langberg; Dorthe Skovgaard; Lars J. Petersen; Jens Bülow; Michael Kjær
- Book ID
- 111118487
- Publisher
- Cambridge University Press
- Year
- 1999
- Tongue
- English
- Weight
- 170 KB
- Volume
- 521
- Category
- Article
- ISSN
- 0022-3751
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📜 SIMILAR VOLUMES
A method to determine the proportions of the major fiber-forming collagens (types I, III, and V) in noncartilaginous human tissues is presented. The procedure relies on direct solubilization of tissue collagen as cyanogen bromide peptides. The peptides are subjected to cation exchange chromatography
In vitro human skin fibroblasts aging was characterized by a continuous increase of collagenase mRNA levels. On the contrary, TIMP‐1 mRNA level decreased only at late passages (> 65% of proliferative life span). Type I and III mRNA levels showed a high variability depending on cell strains studied.