Two types of melanogenesis in monolayer cultures of melanoma cells
โ Scribed by Atsushi Oikawa; Michie Nakayasu; Carolyn Claunch; T.T. Tchen
- Book ID
- 113209855
- Publisher
- Elsevier Science
- Year
- 1972
- Weight
- 359 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0045-6039
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## Abstract A permanent cell line C~2~M of mouse melanoma B16 was highly melanized in a modified Eagle's MEM supplemented with 10% calf serum, when the medium contained 1 mM galactose and 10 mM pyruvate instead of 5.5 mM glucose. The activity of the key enzyme for melanogenesis, tyrosinase (EC 1.14
To elucidate the interaction between melanoma and its matrix, we cultured B16 murine melanoma cells on and in type I collagen gel and evaluated specified functions of melanoma cells; tyrosinase activity and melanin-synthesizing capacity. Proliferation of cells cultured in these environments was mark