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Two-photon fluorescence excitation spectra of aromatic amino acids

✍ Scribed by Aden A. Rehms; Patrik R. Callis

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 577 KB
Volume: 208
Category: Article
ISSN: 0009-2614
DOI: 10.1016/0009-2614(93)89075-s

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✦ Synopsis

Two-photon excited fluorescence excitation spectra using circularly and linearly polarized light are presented for the aromatic amino acids tryptophan, tyrosine (amide form) and phenylalanine in neutral aqueous solution at room temperature. The wavelength range was 440-620 nm, corresponding to 220-310 nm in the UV. In contrast to one-photon (UV) excitation, phenylalanine absorption is about the same as that of tyrosine and both are shifted about 1500 cm-' to higher energy relative to their UV bands. Thus, observing phenylalanine photophysics in proteins may be more feasible with two-photon excitation. Semiempirical theory gives a good account of the results.

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