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Anisotropy spectra of indole and N-acetyl-L-tryptophanamide observed for two-photon excitation of fluorescence

✍ Scribed by Joseph R. Lakowicz; Ignacy Gryczynski; Eva Danielsen; Joan Frisoli

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 511 KB
Volume: 194
Category: Article
ISSN: 0009-2614
DOI: 10.1016/0009-2614(92)86052-j

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✦ Synopsis

We observed the steady state fluorescence anisotropy spectra of indole and N-acetyl-L-tryptophanamide (NATA) resulting from one-(OPE) and two-photon excitation (TPE) offluorescence.

The anisotropy spectra for OPE and TPE are remarkably different, suggesting that the cross sections for relative absorption of the 'L, and 'Lb state are different for one-and two-photon absorption. Frequency-domain time-resolved anisotropy decays confirm the unusual value of the time-zero anisotropies.

Comparison of the OPE and TPE anisotropy spectra should allow a more critical evaluation of the accepted IL,-'Lb model for tryptophan and protein fluorescence.

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PoIarized two-photon fluorescence excitation spectra of indole in hexane, benzimidazole in isopropanoi, and benzimidazole cation in methanol-HaSO.,, all at 0.2 M and 25°C are reported for the excitation range 470-600 nm, the region of their L, and L, bands. Relative two-photon absorptivities are ded