We observed two-color two-photon (2C2P) excitation of indole upon simultaneous illumination at 380 and 760 nm with picosecond pulses from a cavity-dumped dye laser. The emission spectrum of indole with 2C2P excitation was the same as observed for one-photon excitation with an equivalent energy of 25
Two-photon excitation of proteins
โ Scribed by Shou-Ping Jiang; Shao-Hui Lian; Kang-Cheng Ruan; Ling-Kai Hui; Song-Hao Liu; Zhen Zhang; Qun Li
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 257 KB
- Volume
- 104
- Category
- Article
- ISSN
- 0009-2614
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โฆ Synopsis
Fluorescence emission after two-photon excitation at 532 nm by means of a Nd : YAG laser is observed in apohemoglobm, hemoglobm,albumin and tryptophan at room temperature. The experimental results show that the fluorescence of these proteins originates from tryptophan residues_ No fluorescenar of a biphotonic nature could be detected from lysozyme and tyrosine.
๐ SIMILAR VOLUMES
Two-photon excitation of some alkanes by a pulsed nitrogen laser is reported on the basis of the observed emission spectra, decay times and the quadratic dependence of emission intensity on the laser beam intensity.
## Recri\ed 10 Jnnuarv I983 The t\\o-photon excitation spectrum of pgrene md the polarization ratio R have been merrsured up to 52100 A. Eridencc of 3 strong vibronic coupling benreen the first 'Bz,, state and 'Aa states induced by bZu vibwtional modes is presented. By means of n nnd comparison wi