๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Two-phase Matrix-assisted Laser Desorption/Ionization: Matrix Selection and Sample Pretreatment for Complex Anionic Analytes

โœ Scribed by Michael Dale; Richard Knochenmuss; Renato Zenobi

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
138 KB
Volume
11
Category
Article
ISSN
0951-4198

No coin nor oath required. For personal study only.

โœฆ Synopsis

Two-phase matrices, consisting of a binary mixture of solid particulates and a low vapour pressure liquid, are shown to be effective for the production of both positive-ion and negative-ion laser desorption/ionization mass spectra (LDI-MS). It was found that matrix selection can be guided by criteria developed previously for fast-atom bombardment mass spectrometry. Two-phase matrix-assisted (MA)LDI-MS of a variety of complex anionic analytes is presented. Sample pre-treatment using 'drop dialysis', or exposure to an acid-activated Nafionยฎ cation-exchange membrane, substantially enhances the quality of negative-ion mass spectra.

๐Ÿ“œ SIMILAR VOLUMES

Matrix-assisted Laser Desorption/Ionizat
Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Sample Preparation Techniques Designed for Various Peptide and Protein Analytes
โœ Kussmann, Martin; Nordhoff, Eckhard; Rahbek-Nielsen, Henrik; Haebel, Sophie; Ros ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 706 KB

This study encompasses a collection of experiences with regard to numerous matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) sample preparation techniques in terms of their suitability for di โ€ erent peptide and protein analytes. Variants of both established and new sample prep

Matrix effects on selectivities of poly(
Matrix effects on selectivities of poly(ethylene glycol)s for alkali metal ion complexation in matrix-assisted laser desorption/ionization
โœ Hassan Rashidzadeh; Y. Wang; Baochuan Guo ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 109 KB ๐Ÿ‘ 2 views

The effect of the matrix on poly(ethylene glycol) (PEG) cationization was investigated using an equimolar mixture of CsCl and LiCl as cationizing agents. It was observed that for both carboxylic acid and noncarboxylic acid matrices used, PEG cationization by a given alkali metal ion depends on the m

Characterization of Two New Matrices for
Characterization of Two New Matrices for Matrix-assisted Laser Desorption/Ionization Mass Spectrometry
โœ Jian Bai; Xiaoli Liang; Yan-Hui Liu; Yongdong Zhu; David M. Lubman ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 561 KB

Three structurally related compounds, 4-hydroxy-3-methoxyphenylpyruvic acid (HMPPA), indole-3-pyruvic acid (IPA), and indole-3-glyoxylic acid have been evaluated as matrix-assisted laser desorption/ionization (MALDI) matrices. HMPPA and IPA were found to be effective matrices for MALDI-MS analysis o

Optimization of a hydrophobic solid-phas
Optimization of a hydrophobic solid-phase extraction interface for matrix-assisted laser desorption/ionization
โœ Brockman, Adam H.; Shah, Nina N.; Orlando, Ron ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 134 KB

Matrix-assisted laser desorption/ionization (MALDI) probe surfaces derivatized with octadecanethiol can be (C 18 ) used as hydrophobic solid-phase extraction devices to isolate and desalt biopolymers directly on the probe surface. Using quantitative MALDI, it was possible to determine the approximat