Two new glucose-6 phosphate dehydrogenase (G6PD) variants associated with hemolytic anemia: G6PD amman-1 and G6PD amman-2

✍ Scribed by Dr. Naif S. Karadsheh; A. S. Awidi; M. S. Tarawneh

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 385 KB
• Volume: 22
• Category: Article
• ISSN: 0361-8609
• DOI: 10.1002/ajh.2830220209

✦ Synopsis

Two glucose-6-phosphate dehydrogenase (G6PD) variants were investigated. G6PD Amman-1 was partially purified from the red cells of a patient suffering from recurrent jaundice and spontaneous episodic attacks of severe hemolysis in the absence of oxidant drugs, infection, or fava beans. The enzymatic characteristics of G6PD Amman-I were markedly reduced activity, fast eletrophoretic mobility, slightly increased km for NADP, normal km for G-6-P, normal heat stability, normal utilization of substrate analogues 2-deoxy G-6-P and deamino-NADP, and a monophasic pH curve with a peak at 8.5 to 9.3. The second variant, G6PD Amman-2, was partially purified from the red cells of a patient suffering from recurrent jaundice with episodic mild hemolysis caused by infection or unknown factors. G6PD Amrnan-2 characteristics were severely reduced activity, slow electrophoretic mobility, normal krn for NADP, decreased km for G-6-P, decreased heat stability, increased utilization of substrate analogues, and a monophasic pH curve with a narrow peak at pH 9.5. The red cell level of reduced glutathione was markedly decreased with twofold increase in the activity of glutathione reductase in the patient with G6PD Amman-2.