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Molecular abnormality of a unique japanese glucose-6-phosphate dehydrogenase variant (G6PD Kobe) with a greatly increased affinity for galactose-6-phosphate

✍ Scribed by Akira Hirono; Shiro Nakayama; Hisaichi Fujii; Shiro Miwa


Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
197 KB
Volume
45
Category
Article
ISSN
0361-8609

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✦ Synopsis


Abstract

Systematic molecular analysis of a Japanese class 1 glucose‐6‐phosphate dehydrogenase (GGPD) variant (G6PD Kobe) cONA revealed a unique nucleotide substitution (1318 C to T) in exon 11, which predicts a substitution of leucine for phenylalanine at residue 440. This substitution is located in a region surrounding the putative structural NADP‐binding domain. The markedly abnormal kinetics of glucose‐6‐phosphate (GGP) of GGPD Kobe suggest the interaction between both NADP and G6P binding sites. © 1994 Wiley‐Liss, Inc.