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Two methods for construction of internal amplification controls for the detection of Escherichia coli O157 by polymerase chain reaction

✍ Scribed by A. Abdulmawjood; S. Roth; M. Bülte

Book ID
115638500
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
214 KB
Volume
16
Category
Article
ISSN
0890-8508

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Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t