Two-Hybrid Systems: Methods and Protocol
β Stanley Fields, Paul L. Bartel (auth.), Paul N. MacDonald (eds.)
π Library
π
2001
π Humana Press
π English
β¦ LIBER β¦
π
Two Hybrid Technologies: Methods and Protocols
β Scribed by Roman HΓ€user, Thorsten Stellberger, Seesandra V. Rajagopala (auth.), Bernhard Suter, Erich E. Wanker (eds.)
- Publisher
- Humana Press
- Year
- 2012
- Tongue
- English
- Leaves
- 334
- Series
- Methods in Molecular Biology 812
- Edition
- 1
- Category
- Library
β¬ Acquire This Volume
No coin nor oath required. For personal study only.
β¦ Synopsis
Proteinβprotein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell. Therefore, the comprehensive exploration of interactome networks is one of the major goals in systems biology. The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping. Divided into two convenient sections, the first gives a survey of protocols that are currently employed for Y2H high-throughput screens by different expert labs in the field. Rather than detailing the principles of screening, which have been described previously, the focus is on different implementations of Y2H interactome mapping. The second section of the book considers innovative PPI detection methods that have the potential to emerge as alternative high-throughput methodologies. Written in the highly successful Methods in Molecular Biologyβ’ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Two Hybrid Technologies: Methods and Protocols supplies researchers with a comprehensive toolbox for the identification of biologically relevant protein interactions.
β¦ Table of Contents
Front Matter....Pages i-xi
Back Matter....Pages 1-20
....Pages 21-38
β¦ Subjects
Protein Science; Proteomics
π SIMILAR VOLUMES
Two-Hybrid Systems: Methods and Protocol
β Paul N. MacDonald
π Library
π
2001
π Humana Press
π English
Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast
Two-Hybrid Systems: Methods and Protocol
β Stanley Fields, Paul L. Bartel (auth.), Paul N. MacDonald (eds.)
π Library
π
2001
π Humana Press
π English
<p>The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powe
Two-Hybrid Systems: Methods and Protocol
β Stanley Fields, Paul L. Bartel (auth.), Paul N. MacDonald (eds.)
π Library
π
2001
π Humana Press
π English
<p>The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powe
Two-Hybrid Systems: Methods and Protocol
β Paul N. MacDonald
π Library
π
2001
π Humana Press
π English
Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast
Two-Hybrid Systems: Methods and Protocol
β Paul N. MacDonald
π Library
π
2001
π Humana Press
π English
Case Western Research Univ., Cleveland, OH. Reveals how the variety of plasmid vectors and approaches may be optimally deployed, and empowers novices to establish two-hybrid systems in their labs. Includes complete media formulations for the widely used yeast two-hybrid. Expanded-outline format. Har