Two-Hybrid Systems: Methods and Protocol
โ Stanley Fields, Paul L. Bartel (auth.), Paul N. MacDonald (eds.)
๐ Library
๐
2001
๐ Humana Press
๐ English
โฆ LIBER โฆ
๐
Two-Hybrid Systems: Methods and Protocols
โ Scribed by Paul N. MacDonald
- Publisher
- Humana Press
- Year
- 2001
- Tongue
- English
- Leaves
- 336
- Series
- Methods in Molecular Biology
- Edition
- 1
- Category
- Library
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โฆ Synopsis
Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast), to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problem of artifacts and false positives and for identifying the interacting partners in important biological systems, including the SMAD and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly.
๐ SIMILAR VOLUMES
Two-Hybrid Systems: Methods and Protocol
โ Paul N. MacDonald
๐ Library
๐
2001
๐ Humana Press
๐ English
Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast
Two-Hybrid Systems: Methods and Protocol
โ Stanley Fields, Paul L. Bartel (auth.), Paul N. MacDonald (eds.)
๐ Library
๐
2001
๐ Humana Press
๐ English
<p>The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powe
Two-Hybrid Systems: Methods and Protocol
โ Stanley Fields, Paul L. Bartel (auth.), Paul N. MacDonald (eds.)
๐ Library
๐
2001
๐ Humana Press
๐ English
<p>The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powe
Two-Hybrid Systems: Methods and Protocol
โ Paul N. MacDonald
๐ Library
๐
2001
๐ Humana Press
๐ English
Case Western Research Univ., Cleveland, OH. Reveals how the variety of plasmid vectors and approaches may be optimally deployed, and empowers novices to establish two-hybrid systems in their labs. Includes complete media formulations for the widely used yeast two-hybrid. Expanded-outline format. Har
Two-Hybrid Systems: Methods and Protocol
โ Paul N. MacDonald
๐ Library
๐
2001
๐ Humana Press
๐ English
Paul N. MacDonald has assembled a collection of powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast