Two-dimensional gel electrophoresis: recent advances in sample preparation, detection and quantitation
β Scribed by Kathryn S Lilley; Azam Razzaq; Paul Dupree
- Book ID
- 104414596
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 79 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1367-5931
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Two-dimensional gel electrophoresis was used to comprehensively scan the whole genome o f f ) cervical intraepithelial neoplasia (CIN) lesions, 7 cervical squamous cell carcinomas, 1 cervical adenosquamous cell carcinoma, and 2 cervical adenocarcinomas for multiple genetic alterations, such as DNA a
## Abstract We investigate one approach to assess the quantitative variability in twoβdimensional gel electrophoresis (2βDE) separations based on gelβtoβgel variability, sample preparation variability, sample load differences, and the effect of automation on image analysis. We observe that 95% of s