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Detection of genomic alterations in human cervical cancer by two-dimensional gel electrophoresis

✍ Scribed by Jiafan Liu; Yian Wang; Ping Gu; James Patrick; Keith A. Crist; Carol L.K. Sabourin; Gary D. Stoner; Michele F. Mitchell; James D. Fanning; Kitai Kim; Peter J. Goldblatt; Gary J. Kelloff; Charles W. Boone; Ming You

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
796 KB
Volume
63
Category
Article
ISSN
0730-2312

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✦ Synopsis

Two-dimensional gel electrophoresis was used to comprehensively scan the whole genome o f f ) cervical intraepithelial neoplasia (CIN) lesions, 7 cervical squamous cell carcinomas, 1 cervical adenosquamous cell carcinoma, and 2 cervical adenocarcinomas for multiple genetic alterations, such as DNA amplification, chromosome deletion, loss of heterozygosity, and chromosome translocation, as compared with the paired normal tissues. DNA spot analysis of the genomic 2-dimensional gels was performed by a computer color overlay system and by spot recognition software allowing for objective spot comparison and quantitation. Nine spots were found to be amplified in the cervical carcinomas while two amplified spots were detected in the CIN Ill lesions. Fourteen DNA spots were either reduced in their intensity or absent in cervical carcinomas as compared to their normal paired tissues. Reduction of intensity in 6 spots was observed in the 5 CIN 1" lesions. These genetic alterations may represent changes in cancer genes that are associated with human cervicai carcinogenesis. Further characterization of these alterations may be significmt to the understanding of cervical tumorigenesis and to the development of biomarkers for clinical trials in cancer chernopreven-

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