𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Two-dimensional gel electrophoresis of wool intermediate filament proteins

✍ Scribed by Louise N. Paton; Juliet A. Gerrard; Warren G. Bryson

Publisher
Elsevier
Year
2008
Tongue
English
Weight
661 KB
Volume
71
Category
Article
ISSN
1874-3919

No coin nor oath required. For personal study only.

✦ Synopsis

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) methods have been used to provide high-resolution separation of wool intermediate filament proteins (IFPs). An improved method of extraction was developed based on a previously published method.

The improved method for extraction eliminates the use of dialysis and freeze-drying between the extraction and rehydration steps, allowing the extraction and rehydration for the first dimension gel to be achieved in one day. Improvements to the method for maintaining reducing conditions and chaotrope constitution, combined with low %T polyacrylamide gels, allowed the high-resolution separation of the two keratin IFP families and their individual family members. The IFPs were separated to produce a clearly defined spot pattern of higher intensity, with numerous minor spots not previously observed, and a marked improvement in the vertical resolution. Further work to analyse the composition of each of the protein spots has been made much easier by being able to separate the IFPs into discrete spots.

πŸ“œ SIMILAR VOLUMES

Characterisation of wool intermediate fi
Characterisation of wool intermediate filament proteins separated by micropreparative two-dimensional electrophoresis
✍ Ben R. Herbert; Mark P. Molloy; Jun X. Yan; Andrew A. Gooley; Warren G. Bryson; πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 492 KB πŸ‘ 2 views

## Abstract Wool intermediate filament proteins (IFP) are a subclass of the cytokeratins, a group of structural proteins which form intermediate filaments in many cell types. Post‐translational modifications, such as phosphorylation, play an important role in the control of intermediate filament as

Micro-scale two-dimensional polyacrylami
Micro-scale two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins
✍ Lin, Alan ;Collatz, Ekkehard ;Wool, Ira G. πŸ“‚ Article πŸ“… 1976 πŸ› Springer 🌐 English βš– 782 KB

A specially designed apparatus and conditions are described for the rapid analysis of ribosomal proteins by two-dimensional gel electrophoresis on a micro scale. The resolution of proteins in electropherograms is comparable to that obtained with other systems, but because of miniaturization, only 0.

Investigations into charge heterogeneity
Investigations into charge heterogeneity of wool intermediate filament proteins
✍ Louise N. Paton; Juliet A. Gerrard; Warren G. Bryson πŸ“‚ Article πŸ“… 2008 πŸ› Elsevier 🌐 English βš– 782 KB

Genomic studies have shown that there are four abundant type I and type II intermediate filament proteins (IFPs) in wool. When separated using 2D-PAGE, the type I IFPs separated into four clearly defined major rows. The type II IFPs separated into two distinct staggered rows. The large number of spo

Two-dimensional gel analysis of glandula
Two-dimensional gel analysis of glandular keratin intermediate filament phosphorylation
✍ Jiam Liao; Nam-on Ku; Dr. M. Bishr Omary πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 627 KB

Intermediate filament (IF) proteins, in contrast to microtubules and microfilaments, are preferentially expressed in higher eukaryotes, are relatively insoluble, and are made up of a large and diverse family of proteins (reviewed in [1][2][3][4][5]). Aside from the nuclear lamins, members of the cyt

Preparative two-dimensional gel electrop
Preparative two-dimensional gel electrophoresis of membrane proteins
✍ Dr. Christian Pasquali; Irene Fialka; Lukas A. Huber πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 922 KB

## Abstract Electrophoretic techniques, and especially two‐dimensional gel electrophoresis (2‐DE), have provided an indispensable set of tools for the separation of complex protein mixtures as well as for the identification of protein‐protein interactions. Nevertheless, after its introduction more