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Micro-scale two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins

✍ Scribed by Lin, Alan ;Collatz, Ekkehard ;Wool, Ira G.

Publisher
Springer
Year
1976
Tongue
English
Weight
782 KB
Volume
144
Category
Article
ISSN
0026-8925

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✦ Synopsis

A specially designed apparatus and conditions are described for the rapid analysis of ribosomal proteins by two-dimensional gel electrophoresis on a micro scale. The resolution of proteins in electropherograms is comparable to that obtained with other systems, but because of miniaturization, only 0.5 to 1 mug of each protein is required, and the entire procedure, including electrophoresis in both dimensions, and staining and destaining can be completed in 6 to 7 hours.

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