Two-dimensional gel electrophoresis in proteomics: Past, present and future

✍ Scribed by Thierry Rabilloud; Mireille Chevallet; Sylvie Luche; Cécile Lelong

• Book ID: 104027593
• Publisher: Elsevier
• Year: 2010
• Tongue: English
• Weight: 894 KB
• Volume: 73
• Category: Article
• ISSN: 1874-3919
• DOI: 10.1016/j.jprot.2010.05.016

✦ Synopsis

Two-dimensional gel electrophoresis has been instrumental in the birth and developments of proteomics, although it is no longer the exclusive separation tool used in the field of proteomics. In this review, a historical perspective is made, starting from the days where twodimensional gels were used and the word proteomics did not even exist. The events that have led to the birth of proteomics are also recalled, ending with a description of the now wellknown limitations of two-dimensional gels in proteomics. However, the often-underestimated advantages of two-dimensional gels are also underlined, leading to a description of how and when to use two-dimensional gels for the best in a proteomics approach. Taking support of these advantages (robustness, resolution, and ability to separate entire, intact proteins), possible future applications of this technique in proteomics are also mentioned.