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Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry

✍ Scribed by Nakul Mandal; Steffen Heegaard; Jan Ulrik Prause; Bent Honoré; Henrik Vorum

Book ID
107667765
Publisher
BioMed Central
Year
2009
Tongue
English
Weight
616 KB
Volume
12
Category
Article
ISSN
1480-9222

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Separation and relative quantitation of complex protein mixtures remain two of the most challenging aspects of proteomics. Here an advanced technique called fluorescence difference 2-D gel electrophoresis technology (2D-DIGE) has been applied to a model system study of the Escherichia coli proteome