Separation and relative quantitation of complex protein mixtures remain two of the most challenging aspects of proteomics. Here an advanced technique called fluorescence difference 2-D gel electrophoresis technology (2D-DIGE) has been applied to a model system study of the Escherichia coli proteome
Proteome analysis of Pithecellobium dulce seeds using two-dimensional gel electrophoresis and tandem mass spectrometry
β Scribed by Narumon Sawasdipuksa; Lloyd W Sumner; Zhentian Lei; Polkit Sangvanich
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 144 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0022-5142
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Capillary electrophoresis/time-of-flight mass spectrometry(CE/TOFMS) has been used for analysis of in-gel digests of protein spots excised from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE). An off-line purification and preconcentration procedure with a Zip