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Two-dimensional electrophoresis of membrane proteins: A current challenge for immobilized pH gradients

✍ Scribed by Céline Adessi; Christine Miege; Catherine Albrieux; Dr. Thierry Rabilloud


Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
927 KB
Volume
18
Category
Article
ISSN
0173-0835

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Two-Dimensional Electrophoresis of Membr
✍ Mark P. Molloy 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 130 KB

Two-dimensional electrophoresis (2-DE) is a highly resolving technique for arraying proteins by isoelectric point and molecular mass. To date, the resolving ability of 2-DE for protein separation is unsurpassed, thus ensuring its use as the fundamental separation method for proteomics. When immobili