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Two-dimensional 1H-nmr study of synthetic peptides containing the main immunogenic region of the Torpedo acetylcholine receptor

✍ Scribed by M. T. Cung; M. Marraud; I. Hadjidakis; E. Bairaktari; C. Sakarellos; A. Kokla; S. Tzartos

Publisher: Wiley (John Wiley & Sons)
Year: 1989
Tongue: English
Weight: 679 KB
Volume: 28
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.360280141

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✦ Synopsis

synopms

A comparative 'H-NMR spectral study of a synthetic decapeptide containing the main immunogenic region of the Torpedo acetylcholine receptor (AChR; WNPADYGGIK, representing the a67-76 fragment of Torpedo AChR) with four analogous peptides (WNP3-D5YGGIK, WNPAA5YGGIK, WNPADYGGA'K, and WNPD4DYGGV'K) has been carried out in dimethyl sulfoxide.

One-

and two-dimenional nmr experiments [correlated spectroscopy (COSY), relayed COSY, and phase-sensitive nuclear Overhauser enhancement spectroscopy (NOESY)] were performed to obtain complete assignments of the proton resonances. The presence of strong and multiple shortand long-range NOES, and especially a strong long-range NOE between the two Asn'-CaH and Gly7-CaH protons, argues in favor of a rigid folded structure in all five cases. Temperature dependence measurements indicate the existence of three intramolecular interactions involving the As#, Gly', and Lys" amide protons.

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