✦ LIBER ✦
The third-dimensional structure of the complex between an Fv antibody fragment and an analogue of the main immunogenic region of the acetylcholine receptor: A combined two-dimensional NMR, homology, and molecular modeling approach
✍ Scribed by Jens Kleinjung; Marie-Christine Petit; Piotr Orlewski; Avgi Mamalaki; Socrates J. Tzartos; Vassilios Tsikaris; Maria Sakarellos-Daitsiotis; Constantinos Sakarellos; Michel Marraud; Manh-Thong Cung
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2000
- Tongue
- English
- Weight
- 351 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Binding of autoantibodies to the acetylcholine receptor (AChR) plays a major role in the autoimmune disease Myasthenia gravis (MG). In this paper, we propose a structure model of a putative immunocomplex that gives rise to the reduction of functional AChR molecules during the course of MG. The model complex consists of the [G 70 , Nle 76 ] decapeptide analogue of the main immunogenic region (MIR), representing the major antigenic epitope of AChR, and the single chain
Fv fragment of monoclonal antibody 198, a potent MG autoantibody. The structure of the complexed decapeptide antigen [G 70 , Nle 76 ]MIR was determined using two-dimensional nmr, whereas the