Two-Color flow cytometric analysis of monocyte depleted human blood lymphocyte subsets
โ Scribed by Thomas A. Fleisher; Claire Hagengruber; Gerald E. Marti
- Book ID
- 102814911
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 515 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
An interlaboratory flow cytometric comparison of several commercially available human lymphocyte subset reagents was undertaken in three different laboratories. Fresh Hypaque-Ficoll purified blood mononuclear cells were stained at 4ยฐC or 22ยฐC. Direct or indirect surface immunofluorescence was carrie
Background: Human peripheral blood lymphocytes kept in culture after isolation die by an apoptotic process. Detection of apoptosis with labeled Annexin V to demonstrate loss of plasma membrane asymmetry is sensitive, specific, and easy using flow cytometry. This is true in lymphoblastic cell lines w