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Two-color FISH characterization of i(1q) and der(1;16) in human breast cancer cells

✍ Scribed by Nadja Kokalj-Vokac; Anna Alemeida; Michèle Gerbault-Seureau; Bernard Malfoy; Dr. Bernard Dutrillaux

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
526 KB
Volume
7
Category
Article
ISSN
1045-2257

No coin nor oath required. For personal study only.

✦ Synopsis

Two-color fluorescent in situ hybridizations using probes for alphoid (a) and classical satellite (CS) DNAs from chromosomes I and I6 were performed to characterize i( I q), der( I; I6), and complex rearrangements observed in breast cancer cells from fresh tumors and established cell lines. Six of seven i( I q) occurred after breakage in the a I containing region and one of seven was dicentric, with breakage in I p I I .2. The five der( I ; I6)(q I0;p 10) studied appeared to result from a variety of breakpoints involving a I, a 16, CS I, and CS I6 DNAs. All had conserved a I 6 DNA, suggesting a segregation of the der( I ; 16) leading to a loss of 16q and a gain of I q in most cases. One complex rearrangement of chromosome I also appeared to involve chromosome 16, suggesting that a der( I; 16) occurred first, followed by another rearrangement. Both the apparent preferential involvement of constitutive heterochromatin harboring a and CS DNAs and the variety of breakpoints spanning along heterochromatin suggest that the important consequence of the rearrangement is not the breakage per se but the resulting imbalance. Genes Chrorn Concer 7:8-14 (1993).

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