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Two ascorbate peroxidases from broccoli: identification, expression and characterization of their recombinant proteins

โœ Scribed by Fumie Nishikawa; Masaya Kato; Ran Wang; Hiroshi Hyodo; Yoshinori Ikoma; Minoru Sugiura; Masamichi Yano


Book ID
114297877
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
395 KB
Volume
27
Category
Article
ISSN
0925-5214

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Pichia pastoris strains with capability to sequentially express two recombinant proteins under control of the constitutive glyceraldehydes-3-phosphate dehydrogenase (GAP) and the methanol-induced alcohol oxidase (AOX1) promoters have been constructed. The intracellular โค-galactosidase (โค-gal), secre