Tus-mediated arrest of DNA replication in Escherichia coli is modulated by DNA supercoiling
โ Scribed by Majda Valjavec-Gratian; Thomas A. Henderson; Thomas M. Hill
- Book ID
- 110832682
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 498 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0950-382X
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We have previously shown that DNA gyrase of Escherichia coli can promote recombination between heterologous DNAs in a cell-free system (Ikeda et al. 1982). In the present paper, we have studied the nucleotide sequences of several recombination junctions of lambda-pBR322 recombinants and found that t
Escherichia coli rnh mutants were isolated using localized mutagenesis and selective measurements of RNase H activity in mutagenized cell extracts with [3H]poly(rC) X poly(dG) as substrate. RNase H activity in extracts of one mutant, ON152 (rnh-91), was undetectable (less than 0.05% of that of wild-