Turbidimetric assay for penicillin
β Scribed by Green, C. A.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1946
- Tongue
- English
- Weight
- 350 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0368-3494
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π SIMILAR VOLUMES
The hydrolysis of penicillin-V to phenoxyacetic acid and 6-aminopenicillanic acid by the fungal enzyme penicillin-V amidase is of industrial importance since the 6-aminopenicillanic acid produced is an intermediate for semisynthetic penicillins. A rapid colorimetric assay of penicillin-V amidase was
A simple, highly sensitive, and rapid assay for highthroughput screening of penicillin G acylase-producing bacteria is presented. The method is based on the specific release of fluorescent 7-amino-4-methyl-coumarin through cleavage of phenylacetyl-4-methyl-coumaryl-7-amide by penicillin G acylase. T