Tumorigenicity of human lymphoblastoid cell lines, acquired during in vitro culture and associated with chromosome gains

✍ Scribed by J. E. N. Morten; J. H. Hay; C. M. Steel; M. E. Foster; C. L. De; Angelis A. Busuttil

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: French
• Weight: 835 KB
• Volume: 34
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910340406

✦ Synopsis

Tumorigenicity of human lymphoma and lymphoblastoid &ell lines was assessed by their ability to form growing and transplantable masses on subcutaneous inoculation into neonatally thymectomized, Ara-C-protected, totalbody-irradiated mice. By these criteria, I 2 lines of known malignant origin were tumorigenic, I I lymphoblastoid lines, tested after less than one year of in vitro growth, were non-tumorigenic and 8/18 long-established lymphoblastoid lines produced transplantable tumours. All of the long-established lines had acquired karyotypic changes on prolonged culture, the predominant characteristic being a gain of whole chromosomes or of major chromosome segments. None showed the classical 8 I 4 translocation asrociated with Burkitt's lymphoma. Comparisons with nontumorigenic precursors (recovered from liquid nitrogen storage) and with other non-tumorigenic but chromoromally abnormal, lymphoblastoid lines suggest that imbalance of the dosage of genes carried on chromosomes 7.8 and 9 may be important in determining the tumorigenic phenotype.