Acquisition of tumorigenic potential in the human myoepithelial HBL100 cell line is associated with decreased expression of HLA class I, class II and integrin β3 and increased expression of c-myc
✍ Scribed by P. Krief; C. Saint-Ruf; M. Bracke; C. Boucheix; C. Billard; M. Billard; R. Cassingena; C. Jasmin; M. Mareel; B. Azzarone
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- French
- Weight
- 757 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
The human breast cell line HBLIOO acquires the capacity to invade normal tissues and to replace them by proliferation in vitro only at high passage levels (HPL). These cells therefore are a useful model for studying tumor progression in vitro. We have analyzed the expression of cell-surface markers supposed to be involved In the control of the neoplastic process. Quantitative flow cytometry has revealed that: (I) spontaneous expression of HLA class-I antigens strongly decreases in HPL HBLIOO cells vs. LPL cells, which parallels amplification and over-expression of c-myc oncogene; (2) HLA DR antigens can be induced by IFN-gamma in LPL but not in HPL HBLIOO cells; (3) HBLlOO cells secrete a soluble protein factor which specifically inhibits HLA DR induction by IFN-amma even in heterologous cell systems; (4) 50% of LPL HiLlOO cells express integrin p3, whereas HPL HBLIM) cells lose this anti en; (5) this cell line is myoepithelial in origin, since 1005 of HBLIOO cells exhibit the CDlO antigen. Our data stress a role of HLA antigens, of some integrins and of c-myc in the acquisition of malignant potential by myoepithelial mammary cells of the HBLIOO line.