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Tumor necrosis factor-A expression in human primary malignant melanoma and its relationship to tumor infiltration by CD3+ cells

✍ Scribed by Birgitta Sander; Bernt Boeryd

Publisher
John Wiley and Sons
Year
1996
Tongue
French
Weight
825 KB
Volume
66
Category
Article
ISSN
0020-7136

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✦ Synopsis

Optimal conditions for immunohistochemical staining of tumor necrosis factor-a (TNF-a) in paraffin-embedded tissue sections were established to investigate TNF-a expression in human primary malignant melanomas. Seventeen malignant melanomas of the nodular (NMM) and superficially spreading (SSM) subtypes were analyzed. Twelve of these were TNF-a+, while 5 did not stain for the cytokine. To evaluate how TNF-a expression affected the immune response to the tumors, infiltration by CD3+ and mac387+ cells was investigated in NMM.

TNF-a expression seemed to selectively affect the capability of T cells to infiltrate the tumors since TNF-a+ tumors were found to have significantly lower levels of infiltrating CD3+ cells, while there was no difference in numbers of mac387+ cells. These results demonstrate that TNF-a is variably expressed in primary malignant melanoma in vivo and that the T-cell response to TNF-a-expressing NMM is inhibited.

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