Plasminogen activator inhibitor type-2 Is a major protein induced in human fibroblasts and SK-MEL-109 melanoma cells by tumor necrosis factor
✍ Scribed by Barbara A. Pytel; Karsten Peppel; Corrado Baglioni
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 853 KB
- Volume
- 144
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Tumor necrosis factor (TNF) induces the synthesis o i two proteins of M, 42 and 36 kDa in human fibroblasts and SK-MEL-109 melanoma cells. To identify these proteins, a hgtl0 cDNA library was prepared from the mRNA of TNF-treated SK-MEL-109 cells. By screening this library, we found a cDNA that preferentially hybridized to TNF-induced RNA. Hybrid-selected mRNA was translated into a protein of 42 kDa; cDNA sequence analysis followed by a comparison with other known protein sequence5 identified this protein with plasminogen activator inhibitor, type-2 (PAI-2). After removal of TNF, PAIL2 mRNA turned over rapidly, with an apparent half-life of -2.5 h. Addition of dexamethasone increased the turnover of this mRNA, suggesting that the level of PAL2 mRNA could be regulated post-transcriptionally by glucocorticoids. PAI-2 was not secreted, but accumulated in fibroblasts continuously treated with TNF.