Tumor Microenvironment: Study Protocols

✍ Scribed by Constantinos Koumenis, Lisa M. Coussens, Amato Giaccia, Ester Hammond (eds.)

• Publisher: Springer International Publishing
• Year: 2016
• Tongue: English
• Leaves: 300
• Series: Advances in Experimental Medicine and Biology 899
• Edition: 1
• Category: Library

✦ Synopsis

This volume covers the topics presented at the 3rd International Conference on Tumor Microenvironment and Cellular Stress by an international community of researchers. The conference brings together scientists to discuss different cellular and animal models of tumor microenvironment study and identify common pathways that are candidates for therapeutic intervention; stimulate collaboration between groups that are more focused on elucidation of biochemical aspects of stress biology (e.g., HIF regulation) and groups that study the pathophysiological aspects of stress pathways or engaged in drug discovery; and critically evaluate novel targets for imaging or therapeutic intervention that would be of use to the tumor microenvironment community and pharmaceutical industry.

✦ Table of Contents

Front Matter....Pages i-viii
Staining Against Phospho-H2AX (γ-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells....Pages 1-10
Measuring DNA Replication in Hypoxic Conditions....Pages 11-25
Isolation of Proteins on Nascent DNA in Hypoxia and Reoxygenation Conditions....Pages 27-40
In Vivo Interrogation of the Hypoxic Transcriptome of Solid Tumors: Optimizing Hypoxic Probe Labeling with Laser Capture Microdissection for Isolation of High-Quality RNA for Deep Sequencing Analysis....Pages 41-58
Gas Chromatography Coupled to Mass Spectrometry (GC–MS) to Study Metabolism in Cultured Cells....Pages 59-88
Probing Cancer Cell Metabolism Using NMR Spectroscopy....Pages 89-111
Measuring the Impact of Microenvironmental Conditions on Mitochondrial Dehydrogenase Activity in Cultured Cells....Pages 113-120
Measuring Autophagy in the Context of Cancer....Pages 121-143
Methods for Studying Autophagy Within the Tumor Microenvironment....Pages 145-166
Methods: Using Three-Dimensional Culture (Spheroids) as an In Vitro Model of Tumour Hypoxia....Pages 167-196
Isolation of Glioma-Initiating Cells for Biological Study....Pages 197-209
Isolation of Mouse and Human Tumor-Associated Macrophages....Pages 211-229
Mouse Models of Brain Metastasis for Unravelling Tumour Progression....Pages 231-244
Quantification of Lung Metastases from In Vivo Mouse Models....Pages 245-251
A Multimodal Data Analysis Approach for Targeted Drug Discovery Involving Topological Data Analysis (TDA)....Pages 253-268
Efficient Protocol for the Identification of Hypoxic Cell Radiosensitisers....Pages 269-290
Back Matter....Pages 291-297

✦ Subjects

Cancer Research; Molecular Medicine; Immunology