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Tubulin-Colchicine Interactions and Polymerization of the Complex

✍ Scribed by JOSE MANUEL ANDREU; SERGE N. TIMASHEFF


Book ID
118723936
Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
749 KB
Volume
466
Category
Article
ISSN
0890-6564

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Fluorometric assay of tubulin-colchicine
✍ Takao Arai; Tsuneo Okuyama πŸ“‚ Article πŸ“… 1975 πŸ› Elsevier Science 🌐 English βš– 431 KB

A fluorometric assay method for the tubulin-colchicine complex has been developed. This assay method is based on the fact that the binding of colchicine to tubulin leads to a 300-fold enhancement of fluorescence intensity of colchicine at 430 nm. Since the excitation wavelength can be set at 350 nm,