TT Virus Replicates in Stimulated but Not in Nonstimulated Peripheral Blood Mononuclear Cells
✍ Scribed by Luisa F. Mariscal; Juan Manuel López-Alcorocho; Elena Rodrı́guez-Iñigo; Nuria Ortiz-Movilla; Susana de Lucas; Javier Bartolomé; Vicente Carreño
- Book ID
- 112274149
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 278 KB
- Volume
- 301
- Category
- Article
- ISSN
- 0042-6822
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## Abstract TT virus (TTV) loads associated with the peripheral blood cells of seven patients known to carry the virus in plasma were investigated by real‐time PCR. Whereas red cells/platelets were uniformly negative, six and four patients yielded positive peripheral blood mononuclear cells (PBMCs)
## Abstract The TT virus (TTV), a member of a family of human viruses related to the __circoviridae__ viruses, was associated initially with acute and chronic liver diseases. TTV consists of a single‐stranded, circular DNA genome of 3.8 kilobases (kb) and at least three open reading frames (ORFs).
## Abstract In order to determine whether peripheral blood mononuclear cells (PBMC) contain proviral DNA or double‐stranded replicative forms of TT virus (TTV) and to define which cell subset harbors TTV, we analyzed 126 PBMC DNA samples by PCR, using the NG059/NG061/NG063 and the T801/T935 primer