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TT virus: Preferential distribution in CD19+ peripheral blood mononuclear cells and lack of viral integration

✍ Scribed by Qigui Yu; Bruce Shiramizu; Vivek R. Nerurkar; Ningjie Hu; Cecilia M. Shikuma; Marian E. Melish; Karin Cascio; Allison Imrie; Yuanan Lu; Richard Yanagihara

Book ID
102380045
Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
221 KB
Volume
66
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

In order to determine whether peripheral blood mononuclear cells (PBMC) contain proviral DNA or double‐stranded replicative forms of TT virus (TTV) and to define which cell subset harbors TTV, we analyzed 126 PBMC DNA samples by PCR, using the NG059/NG061/NG063 and the T801/T935 primer sets. TTV sequences were detected in nearly 20% (25/126) of PBMC DNA samples. Analysis of PBMC subsets revealed that TTV was preferentially distributed in CD19^+^ cells. TTV DNA was also detected in CD3^+^/CD19^+^ double‐depleted cells but was undetectable in CD3^+^ cells. After degradation of single‐stranded DNA using mung bean nuclease (MBN), TTV could not be detected in previously TTV‐positive PBMC DNA samples nor in DNA from CD19^+^ cells, CD3^+^‐depleted, and CD3^+^/CD19^+^ double‐depleted cells, indicating the lack of TTV DNA integration and the absence of viral double‐stranded replicative intermediates in PBMC or PBMC subset cells. Our data indicate that PBMC and PBMC subsets are not the major sites of TTV replication. J. Med. Virol. 66:276–284, 2002. © 2002 Wiley‐Liss, Inc.

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