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Tryptophan fluorescence properties of cholera toxin upon interacting with ganglioside GD1b

✍ Scribed by M. G. Mestrallet; F. R. Bennun; B. Maggio; F. A. Cumar

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 459 KB
Volume: 12
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490120220

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✦ Synopsis

A blue shift of the tryptophan fluorescence emission spectra of cholera toxin or the B protomer is induced by disialoganglioside GDlb with a capacity similar to that of monosialoganglioside GM,. Both gangliosides were also capable of decreasing or reversing the fluorescence quenching by iodide ion of the toxin. The quenching constants (Ksv) for the toxin fluorescence in absence of gangliosides was 2.8 M-I; in presence of GMI or GDlb, K,, was 0.8 M-' and 0.7 M-I, respectively. Gangliosides GD,, and GTIb were unable to decrease the quenching effect. The results suggest that GDlb induces a perturbation in the tryptophan environment of the toxin molecule similar to that induced by GM I .

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