Solution dynamics of the oligosaccharide moiety of ganglioside GM1: Comparison of solution conformations with the bound state conformation in association with cholera toxin B-pentamer
✍ Scribed by Julia M. Richardson; Mark J. Milton; Steve W. Homans
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 494 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0952-3499
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✦ Synopsis
Abstract
The solution dynamics of the oligosaccharide moiety of ganglioside G~M1~ have been determined by use of a combination of ^1^H rotating frame Overhauser effect measurements and restrained molecular dynamics simulations, It is found that the Galβ1‐3 and NeuNAc moieties which are primarily recognized by cholera toxin both exhibit considerable torsional flexibility about their respective glycosidic linkages. A comparison with the bound state conformation of the ganglioside in association with cholera toxin B‐pentamer, shows that a low energy conformation of the oligosaccharide, which closely approximates the globel minimum, is selected upon binding.