Trypsin induces tumour necrosis factor-α secretion from a human leukemic mast cell line

✍ Scribed by Ok-Hwa Kang; Hyun-Ja Jeong; Dae-Ki Kim; Suck-Chei Choi; Tae-Hyun Kim; Yong-Ho Nah; Hyung-Min Kim; Young-Mi Lee

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 155 KB
• Volume: 21
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/cbf.1014

✦ Synopsis

Abstract

Trypsin activating both proteinase‐activated receptor (PAR) 2 and PAR4 plays an important role in inflammation. We have investigated the potential of trypsin to induce TNF‐α secretion from the human leukemic mast cell line (HMC‐1). HMC‐1 cells co‐express both PAR2 and PAR4, and their agonist trypsin signals to HMC‐1 cells. Trypsin (100 nm), SLIGKV‐NH~2~ (100 μm, corresponding to the PAR2 tethered ligand), or GYPGQV‐NH~2~ (100 μm, corresponding to the PAR4 tethered ligand) induced tumour necrosis factor (TNF)‐α secretion from HMC‐1 cells. TNF‐α secretion by trypsin was significantly blocked by pretreatment with 50 μm PD098059, MEK‐1 inhibitor. Furthermore, trypsin stimulated the activation of extracellular signal‐regulated kinase (ERK) in HMC‐1 cells without any detectable activation of c‐Jun N‐terminal kinase (JNK) and p38 MAP kinase homologue. These results show that trypsin may induce TNF‐α secretion following activation of ERK via both PAR2 and PAR4 on HMC‐1 cells. Copyright © 2003 John Wiley & Sons, Ltd.