A convenient and rapid procedure for ext
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K.C. Gupta; M.W. Taylor
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Article
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1977
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Elsevier Science
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English
โ 445 KB
A convenient and rapid procedure has been developed to extract RNA polymerase II quantitatively from cultured mammalian cells. The procedure was successfully employed on HeLa, CHO, L, and MDBK cells. The easy extraction of RNA polymerase II by freeze-thaw of cells in a buffer of low ionic strength s