Tritium labeled prostacyclin sodium salt

✍ Scribed by Richard S. P. Hsi; Wayne T. Stolle; James P. McGrath; Douglas R. Morton

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: French
• Weight: 412 KB
• Volume: 18
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580181007

✦ Synopsis

Reduction o f PGD2 methyl e s t e r w i t h sodium b o r o t r i t i d e a f f o r d e d [ l l ~-~H ] o r o s t a q l a n d i n F p (PGF~Lx) methyl e s t e r (za) i n 57% radiochemical y i e l d . [115-HIPGFza methyl e s t e r was converted uia (5R, 6R)-and (5S, 6S)-5-iodo-[ l l ~-~H ] p r o s t a q l a n d i n 1 1 methyl e s t e r ( 3 ) t o [l 15-3H]p r o s t a c y c l i n methyl e s t e r (4). C a r e f u l l y c o n t r o l l e d h y d r o l y s i s o f 4 Droduced c r y s t a l 1 i n e [115-3H]PG12 sodium s a l t which was shown t o be >95% pure by means o f h i q h performance l i q u i d chromatographic (HPLC) a n a l y s i s . The o v e r a l l y i e l d from [11B-3H]PGF2n methyl e s t e r t o [llB-3H]PG12 sodium s a l t was 53%. [11B-3H]PG12 sodium s a l t w i t h a s p e c i f i c a c t i v i t y o f 502 pCi/mq has o n l v l i m i t e d radiochemical s t a b i l i t y . I t s radiochemical D u r i t y d e c l i n e d 15% a f t e r t h r e e weeks o f storage a t -20°C i n t h e c r y s t a l l i n e s t a t e .