TRISODIUM PHOSPHONOFORMATE INHIBITS WOODCHUCK HEPATITIS VIRUS ASSOCIATED DNA POLYMERASE
β Scribed by Nordenfelt, Erik ;Werner, Barbara
- Book ID
- 114821509
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 2009
- Weight
- 163 KB
- Volume
- 88B
- Category
- Article
- ISSN
- 0304-131X
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## Abstract The effects of heat, sodium hypochlorite, diethyl ether, and ethyl alcohol on the activity of DNA polymerase (DNAβP) associated with hepatitis B virsus (HBV) in serum were evaluated. The response of DNAβP to heating at 60Β°C for 15, 30, 45, 60, 90, 120, 180, and 240 minutes was studied a
This paper describes an assay for hepatitis B DNA polymerase which is based on the use of phosphonoformic acid (PFA), a known inhibitor of the viral enzyme, with particulate fractions prepared from blood plasma or serum. The assay makes possible the measurement of the viral enzyme activity in the pr