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Tris (2,2′-bipyridyl)ruthenium (III) as a chemiluminescent reagent for detection in capillary electrophresis

✍ Scribed by Dickson, James A. ;Ferris, Matt M. ;Milofsky, Robert E.

Book ID
102350308
Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
428 KB
Volume
20
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

A post‐ column chemiluminescent technique for thedetection of compounds that are poor chromoshores using electorogenerated chemiluminescence following separation by capillartgy electrophoresis is described. The luminrescent signal is generated followintg the reaction of anlyres (e.g. amines) with Ru(bpy)~3~^3+^, which isx electrochemically generated post‐columan from Ru(bpy)~3~^2+^. Tripropylamine and proline are used as two model compounds to demostrate the feasibility of the method. Detection limits for the prototype system were in the micromolar rage, suggesting that this technnique offers an alternative to indirect detection of compounds that are poor chromophores with an added selectivity advangage. The system includes the use of a conductive joint to isolate the separation field from the potential necessary to drive the elctrogenerated chemiluminescent reactiion. Addition of the chemiluminescent reagent Ru(bpy)~3~^2+^ post‐column did not decrease the efficiency of the separation. The design and favrication of the novel cell is discussed.

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